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Organ-specific differences in the ratio of alternatively spliced chorismate synthase (LeCS2) transcripts in tomato

✍ Scribed by Jörn Görlach; Hanns-R. Raesecke; Gernot Abel; Rolf Wehrli; Nikolaus Amrhein; Jürg Schmid


Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
814 KB
Volume
8
Category
Article
ISSN
0960-7412

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✦ Synopsis


The primary transcript of one of the two chorismate synthase genes (LeCS2) of tomato is differentially processed due to an alternative splicing of the third intron. A novel observation was made when the abundances of the two LeCS2‐specific transcripts in different organs were analysed. The ratio of these two transcripts differs in RNA populations from different organs. Possible explanations for this finding and its potential physiological impact for plant metabolism are discussed.


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The abundance of transcripts specific for several tomato (Lycopersicon esculcntum L.) genes encoding enzymes of the prechorismate pathway was analyzed in different organs of mature plants utilizing a dot-blot assay which was developed for this purpose. The transcript levels were determined for two 3