Abundance of transcripts specific for genes encoding enzymes of the prechorismate pathway in different organs of tomato (Lycopersicon esculentumL.) plants
✍ Scribed by Jörn Görlach; Jürg Schmid; Nikolaus Amrhein
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 996 KB
- Volume
- 193
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
The abundance of transcripts specific for several tomato (Lycopersicon esculcntum L.) genes encoding enzymes of the prechorismate pathway was analyzed in different organs of mature plants utilizing a dot-blot assay which was developed for this purpose. The transcript levels were determined for two 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (EC 4.1.2.15), one shikimate kinase (EC 2.7.1.71), one 5-enolpyruvylshikimate 3-phosphate synthase (EC 2.5.1.19), and two chorismate synthase (EC 4.6.1.4) genes in leaves, cotyledons, stems, roots, and flowers. These organ-specific expression patterns were compared with that of the phenylalanine ammonia-lyase (EC 4.3.1.5) gene family of tomato.