✦ LIBER ✦

Ordered replication of DNA sequences: Synthesis of mouse satellite and adjacent main band sequences

✍ Scribed by Douglas C. Dooley; Harvey L. Ozer

Book ID: 102883221
Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 891 KB
Volume: 98
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040980310

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The replication of mouse satellite DNA was delayed when synchronized 3T3 cells were exposed to low concentrations of hydroxyurea during S phase. It appears that the onset of satellite replication is not a time dependent event, but instead requires that a certain amount of main band DNA be synthesized first. Using hydroxyapatite chromatography and S~1~ nuclease digestion, a procedure was developed to quantitate the synthesis of both satellite and neighboring main band sequences. The replication kinetics of satellite determined by this method agree with previous estimates. Main band sequences adjacent to satellite appear to replicate in concert with satellite DNA. The results are discussed and related to the limitations of the techniques utilized.

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