Optofluidic chip for single cell trapping and stretching fabricated by a femtosecond laser

✍ Scribed by Francesca Bragheri; Lorenzo Ferrara; Nicola Bellini; Krishna C. Vishnubhatla; Paolo Minzioni; Roberta Ramponi; Roberto Osellame; Ilaria Cristiani

• Book ID: 102290698
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 365 KB
• Volume: 3
• Category: Article
• ISSN: 1864-063X
• DOI: 10.1002/jbio.201000011

✦ Synopsis

Abstract

The authors present the design and optimization of an optofluidic monolithic chip, able to provide optical trapping and controlled stretching of single cells. The chip is fabricated in a fused silica glass substrate by femtosecond laser micromachining which can produce both optical waveguides and microfluidic channels with great accuracy. A new fabrication procedure adopted in this work allows the demonstration of microchannels with a square cross‐section, thus guaranteeing an improved quality of the trapped cell images. Femtosecond laser micromachining emerges as a promising technique for the development of multifunctional integrated biophotonic devices that can be easily coupled to a microscope platform, thus enabling a complete characterization of the cells under test. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)