Optimum tissue culture conditions for selection of resistance toPhytophtora cinnamomiin pine callus tissue

✍ Scribed by J. C. Jang; F. H. Tainter

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 339 KB
• Volume: 9
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00232102

✦ Synopsis

The present experimentation compared the best nutrient medium, temperature, and growth hormones for callus induction and growth of various pine species from different seed sources with their effect on growth of Phytophthora cinnamomi. Callus tissues maintained on a modified Murashige and Skoog medium with 10(-5)M 2,4-D at 26°C in the dark optimized the expression of differential resistance when inoculated with hyphae of P. cinnamomi. High concentration of 2,4-D (5×10(-5)M) inhibited growth of P. cinnamomi.