✦ LIBER ✦

Multiplication of tobacco mosaic virus in tobacco callus tissues and in vitro selection for viral disease resistance

✍ Scribed by Hideyoshi Toyoda; Kazuyuki Chatani; Yoshinori Matsuda; Seiji Ouchi

Publisher: Springer
Year: 1989
Tongue: English
Weight: 897 KB
Volume: 8
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00269042

No coin nor oath required. For personal study only.

✦ Synopsis

Tobacco mosaic virus-resistant tobacco was selected in vitro using callus tissues induced from axillary buds of systemically infected tobacco plants. Callus lines in which the virus was continuously multiplying were first isolated and redifferentiated into shoots. By the procedure, non-diseased, healthy shoots were successfully isolated from diseased shoots, which showed typical mosaic symptoms of the virus, and regenerated into intact plants.These regenerated plants showed resistance to virus inoculation, and selfed progeny of virus-resistant regenerants segregated the resistance and susceptibility according to the Mendelian system.

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