Shake flasks were successfully employed for the cultivation of Spodoptera frugiperda (Sf-9) insect cells and for the production of beta-galactosidase, a recombinant model protein, utilizing the baculovirus expression vector system. The culture doubling time and maximal cell density were 20 h and 5 x
Optimizing the baculovirus expression vector system
β Scribed by Richard B. Hitchman; Elisabetta Locanto; Robert D. Possee; Linda A. King
- Book ID
- 116987303
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 223 KB
- Volume
- 55
- Category
- Article
- ISSN
- 1046-2023
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A mathematical model has been developed that predicts the cell population dynamics and production of recombinant protein and infective extracellular virus progeny by insect cells after infection with baculovirus in batch suspension culture. Infection in the model is based on the rate of virus attach
The inability to infect insect cell cultures at the highest achievable cell densities has imposed major limitations to both the fundamental understanding of the Baculovirus Expression Vector System (BEVS) as well as full exploitation of its potential productive capacity for recombinant (β€-galAcNPV)