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Optimization of protein-production by the baculovirus expression vector system in shake flasks

✍ Scribed by Ronen Neutra; Ben-Zion Levi; Yuval Shoham

Publisher
Springer
Year
1992
Tongue
English
Weight
511 KB
Volume
37
Category
Article
ISSN
1432-0614

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✦ Synopsis

Shake flasks were successfully employed for the cultivation of Spodoptera frugiperda (Sf-9) insect cells and for the production of beta-galactosidase, a recombinant model protein, utilizing the baculovirus expression vector system. The culture doubling time and maximal cell density were 20 h and 5 x 10(6) cells/ml respectively. The optimal liquid volumes for flasks rotating at 100 rpm were 25-40% of the flask total volume. Enzyme production (about 600 mg/l) was best at a multiplicity of infection of between 1 and 20 and at a cell density at time of infection of 0.7 x 10(6) cells/ml. At a rotation speed of 100 rpm, Pluronic F-68 had no effect on growth and enzyme production.

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