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Optimizing separation conditions for proteins and peptides using imaged capillary isoelectric focusing

✍ Scribed by Jiaqi Wu; Shun-Cheng Li; Arthur Watson

Book ID
108341483
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
281 KB
Volume
817
Category
Article
ISSN
1873-3778

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## Abstract A comprehensive two‐dimensional (2‐D) separation system, coupling capillary reverse‐phase liquid chromatography (cRPLC) to capillary isoelectric focusing (CIEF), is described for protein and peptide mapping. cRPLC, the first dimension, provided high‐resolution separations for salt‐free