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Two-Dimensional Microcolumn Separation Platform for Proteomics Consisting of On-Line Coupled Capillary Isoelectric Focusing and Capillary Electrochromatography. 1. Evaluation of the Capillary-Based Two-Dimensional Platform with Proteins, Peptides, and Human Serum

โœ Scribed by Zhang, Minquan; El Rassi, Ziad


Book ID
118748094
Publisher
American Chemical Society
Year
2006
Tongue
English
Weight
306 KB
Volume
5
Category
Article
ISSN
1535-3893

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Two-dimensional separation system by on-
โœ Juan Wei; Xue Gu; Yan Wang; Yi Wu; Chao Yan ๐Ÿ“‚ Article ๐Ÿ“… 2010 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 284 KB ๐Ÿ‘ 2 views

A novel on-line 2-D system was developed for peptide and protein mapping. The system combines capillary IEF (cIEF) with pressurized CEC (pCEC) using a micro-injection valve as the interface. Sample fractions, which were focused and separated in the firstdimension cIEF based on their differences in p