✦ LIBER ✦

Optimizing coupled enzyme assays

✍ Scribed by W.W. Cleland

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 295 KB
Volume: 99
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90055-1

No coin nor oath required. For personal study only.

✦ Synopsis

Equations are given for minimizing the cost and calculating the maximum practical rates for coupled assays when two or more coupling enzymes are involved, and a lag of a predetermined size can be tolerated. The lag caused by obligatory mutarotation of an intermediate is also calculated.

📜 SIMILAR VOLUMES

Optimizing enzyme assays with one or two

Optimizing enzyme assays with one or two coupling enzymes

✍ F. García-Carmona; F. García-Cánovas; J.A. Lozano 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 355 KB

The cost of assays using one or two coupling enzymes is optimized by using equations to calculate the minimum amount(s) of enzyme(s) which should be used to obtain a given time (r& in which 99% of the rate V, of the first reaction is obtained. Using two coupling enzymes and given a value of rae, the

Enzyme-Coupled Assays for Proteases

✍ Brian E. Cathers; John V. Schloss 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 104 KB

A coupled spectrophotometric enzyme assa

A coupled spectrophotometric enzyme assay for methyltransferases

✍ Vincenzo Zappia; Patrizia Galletti; Maria Cartenì-Farina; Luigi Servillo 📂 Article 📅 1974 🏛 Elsevier Science 🌐 English ⚖ 458 KB

A coupled optical enzyme assay for phosp

A coupled optical enzyme assay for phosphopentomutase

✍ Maria Grazia Tozzi; Roberta Catalani; Pier Luigi Ipata; Umberto Mura 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 401 KB

Published assays for phosphopentomutase activity are based on acid lability differences between ribose l-phosphate and ribose S-phosphate. The present work describes a new method in which the isomerization of ribose 5-phosphate to ribose l-phosphate is followed spectrophotometrically at 265 nm by co

Fluorescent Coupled Enzyme Assays for d-

Fluorescent Coupled Enzyme Assays for d-Alanine: Application to Penicillin-Binding Protein and Vancomycin Activity Assays

✍ William G. Gutheil; Miglena E. Stefanova; Robert A. Nicholas 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 96 KB

## D-Alanine (D-Ala ) is a ubiquitous constituent of bacterial cell walls. Assays for D-Ala can be used to investigate several aspects of cell wall biosynthesis and the effects of antibiotics on this process. High-sensitivity fluorescent assays for D-Ala were developed in a microtiter plate format

ChemInform Abstract: Enzyme Assays

✍ Jean-Louis Reymond; Viviana S. Fluxa; Noelie Maillard 📂 Article 📅 2009 🏛 John Wiley and Sons ⚖ 15 KB