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A coupled optical enzyme assay for phosphopentomutase

✍ Scribed by Maria Grazia Tozzi; Roberta Catalani; Pier Luigi Ipata; Umberto Mura

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
401 KB
Volume
123
Category
Article
ISSN
0003-2697

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✦ Synopsis

Published assays for phosphopentomutase activity are based on acid lability differences between ribose l-phosphate and ribose S-phosphate. The present work describes a new method in which the isomerization of ribose 5-phosphate to ribose l-phosphate is followed spectrophotometrically at 265 nm by coupling it with the following two-stage enzymatic conversion: ribose l-phosphate + adenine S phosphate + adenosine (adenosine phosphorylase); adenosine + HZ0 -) inosine + NH3 (adenosine deaminase). The method has been used to show some properties of Escherichia coli phosphopentomutase.

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