A coupled enzyme assay for aldose 1-epimerase
β Scribed by Michael K. Weibel
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 271 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0003-2697
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The development of a coupled enzyme assay for the determination of isopenicilhn N synthetase activity in purified extracts from Cephalosporium acremonium was described. Isopenicillin N formed from its precursor, 8-(t-cr-aminoadipyl)-t-cysteinyl-o-valine (ACV), by the synthetase was hydrolyzed by @-l