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Optimizing a method of protein extraction for two–dimensional electrophoretic separation of proteins from planarians (Platyhelminthes, Turbellaria)

✍ Scribed by Dr. Joan Collet; Jaume Baguñà


Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
666 KB
Volume
14
Category
Article
ISSN
0173-0835

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Two-dimensional electrophoretic separati
✍ Norbeck, Joakim; Blomberg, Anders 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 346 KB 👁 2 views

The proteome of the yeast Saccharomyces cerevisiae was analysed by two-dimensional (2D) polyacrylamide gel electrophoresis utilizing a non-linear immobilized pH gradient (3-10) in the first-dimensional separation. Cells were labelled by [ 35 S]methionine incorporation in the respiro-fermentative pha