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Optimized Analysis of Intracellular Adenosine and Guanosine Phosphates in Escherichia coli

โœ Scribed by Sylke Meyer; Naruemol Noisommit-Rizzi; Matthias Reuss; Peter Neubauer

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
271 KB
Volume
271
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

To investigate the intracellular concentrations of adenosine phosphates in Escherichia coli, especially during bioreactor cultivations, a method that enables reproducible determination of adenosine phosphates in culture solutions containing at least 0.25 g dry cell weight/L has been developed. The detection limits of AMP, ADP, and ATP were found to be as low as 1 pmol. The method involves fast sampling, instantaneous inactivation of cell metabolism, extraction of nucleotides, and quantitative analysis by ion-pair reversedphase HPLC.

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## KEY WORDS Allyl isothiocyanate (AITC) has been used as a food additive for paste-type wasallyl isothiocyanate abi (Japanese horseradish) and karashi (Japanese mustard) almost only in Japan, DNA damage even though it has been reported to be carcinogenic and mutagenic. In the reactive oxygen spec