DNA damage and intracellular generation of reactive oxygen species by allyl isothiocyanate in Escherichia coli
✍ Scribed by Yusuke Yonezawa; Katsuhiro Nishikawa; Hajime Nishioka
- Publisher
- John Wiley and Sons
- Year
- 1999
- Weight
- 69 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1095-1539
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✦ Synopsis
KEY WORDS
Allyl isothiocyanate (AITC) has been used as a food additive for paste-type wasallyl isothiocyanate abi (Japanese horseradish) and karashi (Japanese mustard) almost only in Japan, DNA damage even though it has been reported to be carcinogenic and mutagenic. In the reactive oxygen species present paper, the capacities for DNA damage and the intracellular generation 'rec-lac assay' of reactive oxygen species (ROS) by AITC were examined by a 'rec-lac assay' and 'kat-sod assay' a 'kat-sod assay' by using E. coli mutants lacking DNA repair capacities and ROS scavenging enzymes, respectively. Both assays were developed by Nishioka et al. and consist of a streak test, a survival test and a -galactosidase test. The results show strong DNA damaging capacity, and intracellular generation of ROS by AITC. These results indicate the possibility that intracellular ROS performs some role in DNA damage in bacterial cells treated with AITC.