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Optimization of xylanase production byStreptomycessp. P12-137 using response surface methodology and central composite design

✍ Scribed by Gigi Coman; Gabriela Bahrim

Book ID
107689940
Publisher
Springer-Verlag
Year
2011
Tongue
English
Weight
570 KB
Volume
61
Category
Article
ISSN
1590-4261

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✦ Synopsis

Response surface methodology and central composite design were used to optimize a biosynthesis medium for the production of xylanases by Streptomyces sp. P12-137 in submerged fermentation culture at pHΒ 5.0, with wheat bran as substrate. The three variables involved in this research were the wheat bran, potassium nitrate and xylose concentrations. Statistical analysis of the results showed that, in the range studied, xylose and potassium nitrate concentrations had a significant effect on xylanase production. The optimized biosynthesis medium contained (in %, w/v): wheat bran 1.0, KNO~3~ 1.0, xylose 0.5. This medium resulted in a 3-fold increased level of the xylanase (27.77 UA/ml) production compared to the initial level (8.30 UA/ml) after 120Β h of fermentation, whereas the value predicted by the quadratic model was 26.45 UA/ml.

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