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Optimization of trehalose production by a novel strain Brevibacterium sp. SY361

✍ Scribed by Lei Wang; Rui Huang; Guanbin Gu; Hongying Fang

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
190 KB
Volume
48
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

Trehalose production by a novel strain of Brevibacterium sp__.__ SY361 was optimized in submerged fermentation. Different chemical and physical parameters such as carbon and nitrogen sources, inoculum level, initial pH, incubation temperature, aeration and time‐course of fermentation, were studied in order to increase trehalose productivity. An optimal production medium containing 3% (w/v) glucose, 0.9% (v/v) corn steep liquor, 0.5% (w/v) KH~2~PO~4~ and 0.4% (w/v) MgSO~4~Β·7 H~2~O was found suitable for trehalose production. An optimal volume of medium in a 500 ml flask was 80 ml. The optimal levels of other parameters were 4.0% (v/v) of inoculum, initial pH of 6.0, incubation temperature of 28–32 Β°C and time‐course of 60 h. Optimized parameters gave a maximum trehalose of 12.2 mg/ml with a conversion rate of 58.4%. (Β© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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