Optimization of the eluent delivery system for micro size-exclusion chromatography

✍ Scribed by Maxim L. Alexandrov; Boris G. Belenkii; Vladimir A. Gotlib; Jeno E. Kever

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 324 KB
• Volume: 4
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220040503

✦ Synopsis

Abstract

The problems of microcolumn size‐exclusion chromatography and its instrumentation are considered. It is shown that the reproducibility of retention times of a micro‐gel chromatograph depends on the stability of eluent delivery by a syringe micropump. It is possible to increase this stability and to suppress the relaxation processes related to the changes in hydrodynamic column resistance and in the solvent temperature in the pump chamber.

In order to suppress instability when columns of 0.5‐mm inner diameters are used, the volume of the eluent delivery system should be as small as possible (Vp ⩽ 1 mL) and the system should be thermostatted at ΔT 〈P∞(α/β)δ where P ∞ is the pressure under conditions of constant pump pressure, α and β are the coefficients of thermal expansion and compressibility of the liquid, and δ is the permissible retention time error (Δt~R~/t~R~).