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Optimization of the EcoRI*-activity of EcoRI endonuclease

✍ Scribed by Hubert Mayer


Book ID
115908573
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
950 KB
Volume
90
Category
Article
ISSN
0014-5793

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Two activated matrices have been developed to determine whether immobilization chemistry can be used to orient proteins on a support. Restriction endonuclease EcoRI from Escherichia coli RY13 (E.C.3.1.23.13) was used as a model in these studies. Thiol-activated Sephadex G-10 was used to couple the E