A rapid procedure for purification of EcoRI endonuclease
✍ Scribed by János Sümegi; Danielle Breedveld; paul Hossenlopp; Pierre Chambon
- Book ID
- 118312173
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 450 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0006-291X
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📜 SIMILAR VOLUMES
A two step method for the purification of restriction endonuclease Eco RI was developed. The first step involved the purification of the enzyme on Cibacron Blue-F3GA-agarose column, followed by a hydroxyapatite column. The enzyme was homogeneous on SDS-PAGE and completely free from contaminating nuc
The properties of two plasmids coding for the CcoRI restriction and modification enzymes are described. Both plasmids are non auto-transferring (NTP) but can be mobilised by transfer factors. Strains carrying NTP13 produce colicin E1 and the EcoRI enzymes. This plasmid has a molecular weight of 6 X