Optimization of the cultivation medium for natamycin production by Streptomyces natalensis

✍ Scribed by Mohamed A. Farid; Hesham A. El-Enshasy; Ahmed I. El-Diwany; El-Sayed A. El-Sayed

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 149 KB
• Volume: 40
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/1521-4028(200007)40:3<157::aid-jobm157>3.0.co;2-1

✦ Synopsis

The effects of certain nutrients on natamycin production by Streptomyces natalensis in submerged batch culture were studied. The production of this antibiotic required glucose in the cultivation medium with a concentration of 20 g/l. On the other hand, the highest antibiotic production was obtained in a cultivation medium containing 0.05 g/l of potassium dihydrogen phosphate. Further increase in phosphate concentration resulted in a significant increase in biomass concomitant with lower antibiotic production. Among different N-sources tested, only ammonium sulphate, sodium nitrate and beef extract were the suitable nitrogen sources in supporting the antibiotic production. Furthermore, a mixture of beef extract and yeast extract (8 g/l and 2 g/l, respectively) exhibited a synergistic effect in enhancing the natamycin production reaching about 1.5 g/l.