✦ LIBER ✦
Continuous semi-solid cultivation for the production of cellulase by Trichoderma reesei mutants using a polyurethane foam carrier and a liquid medium
✍ Scribed by Z. Targonski; J. Pielecki
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 413 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0138-4988
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✦ Synopsis
The production of cellulase was investigated in semi-solid state culture using the immobilized mycelium of Trichoderm reesei mutants on polyurethane foam impregnated with lactose medium. An extremely high value of about 2.6 FPUIml was reached after the cultivation of T. reesei D-78085 on a 0.5% lactose medium in continuous culture at a pH medium of 4.0 when a bioreactor with vertical polyurethane foam plates was used. The enzyme yield on lactose was 520 FPU/g of lactose metabolized in comparison with 160 FPU/g using a stirred tank bioreactor.