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Optimization of technical conditions for the transformation of Pediococcus acidilactici P60 by electroporation

✍ Scribed by M. Carmen Rodríguez; M. Teresa Alegre; Juan M. Mesas

Book ID: 113855315
Publisher: Elsevier Science
Year: 2007
Tongue: English
Weight: 216 KB
Volume: 58
Category: Article
ISSN: 0147-619X
DOI: 10.1016/j.plasmid.2006.12.005

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Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-f