✦ LIBER ✦

Optimization of sample preparation for plasma desorption mass spectrometry of peptides and proteins using a nitrocellulose matrix

✍ Scribed by Per F. Nielsen; Klaus Klarskov; Peter Højrup; Peter Roepstorff

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Volume: 17
Category: Article
ISSN: 1076-5174
DOI: 10.1002/bms.1200170503

No coin nor oath required. For personal study only.

✦ Synopsis

Molecular ion yields and spectrum quality in plasma desorption mass spectrometry of peptides and proteins have been studied as a function of the method of sample preparation. Parameters like matrix preparation, pH of the sample solution, method of applying the sample on the matrix and sample quantity have been studied. The best matrix was found to be nitrocellulose prepared by electrospraying. Various techniques for applying the sample result in different sensitivity. The highest sensitivity is obtained using the spin-drying technique, which results in highquality spectra with 1-2 pmol of trypsin (mol. wt 23,463). The results obtained using different sample prep aration techniques are discussed and directions for an optimal procedure are given.

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