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Optimization of nuclear localization signal for nuclear transport of DNA-encapsulating particles

✍ Scribed by Akiko Eguchi; Hiroyuki Furusawa; Akitsugu Yamamoto; Teruo Akuta; Mamoru Hasegawa; Yoshio Okahata; Mahito Nakanishi

Book ID
113690881
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
794 KB
Volume
104
Category
Article
ISSN
0168-3659

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We have studied the nuclear transport of H1 histones using the digitonin permeabilization assay system in order to establish the transport requirements for H1 translocation to the nucleus. Using HeLa cells and fluorescencelabeled calf thymus H1, we show that the H1 nuclear transport in permeabilized