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A peptide nucleic acid–nuclear localization signal fusion that mediates nuclear transport of DNA

✍ Scribed by Brandén, Lars J.; Mohamed, Abdalla J.; Smith, C. I. Edvard

Book ID
109484368
Publisher
Nature Publishing Group
Year
1999
Tongue
English
Weight
306 KB
Volume
17
Category
Article
ISSN
1087-0156

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We have studied the nuclear transport of H1 histones using the digitonin permeabilization assay system in order to establish the transport requirements for H1 translocation to the nucleus. Using HeLa cells and fluorescencelabeled calf thymus H1, we show that the H1 nuclear transport in permeabilized