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Optimization of electroporation for transfection of mammalian cell lines

✍ Scribed by Grai L. Andreason; Glen A. Evans


Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
819 KB
Volume
180
Category
Article
ISSN
0003-2697

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A simple procedure to obtain plasmid preparations, suitable for transfecting mammalian cell lines using a calcium phosphate co-precipitation technique, is described. The protocol is based on the purification of plasmid DNA by double gel-filtration chromatography on Sephacryl S-1000 and additional sl