✦ LIBER ✦
A simple procedure to obtain plasmids suitable for transfecting mammalian cell lines
✍ Scribed by L. López-Cánovas; M. Rodríguez; A. M. Riverón Rojas
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 138 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1573-0972
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✦ Synopsis
A simple procedure to obtain plasmid preparations, suitable for transfecting mammalian cell lines using a calcium phosphate co-precipitation technique, is described. The protocol is based on the purification of plasmid DNA by double gel-filtration chromatography on Sephacryl S-1000 and additional slight modifications to the original transfection procedure. The purity of plasmid preparation was verified by analytical methods. The resulting preparation efficiently transfected NIH-3T3 cells.