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Optimization of dissimilation of glycerol to 1,3-propanediol by Klebsiella pneumoniae in one- and two-stage anaerobic cultures

✍ Scribed by Zhi-Long Xiu; Bing-Hui Song; Zong-Tao Wang; Li-Hua Sun; En-Min Feng; An-Ping Zeng

Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
240 KB
Volume
19
Category
Article
ISSN
1369-703X

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Kinetic, dynamic, and pathway studies of glycerol metabolism by Klebsiella pneumoniae in anaerobic continuous culture: III. Enzymes and fluxes of glycerol dissimilation and 1,3-propanediol formation
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The initial steps of glycerol dissimilation and 1,3-propanediol (1,3-PD) formation by Klebsiella pneumoniae anaerobically grown on glycerol were studied by quantifying the in vitro and in vivo activities of enzymes in continuous culture under conditions of steady state and oscillation and during tra

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## Abstract **BACKGROUND:** Glycerol dehydrogenase [EC.1.1.1.6] and 1,3‐propanediol oxidoreductase [EC.1.1.1.202] were proved to be two of the key enzymes for glycerol conversion to 1,3‐propanediol in __Klebsiella pneumoniae__ under anaerobic conditions. For insight into their significance on 1,3‐p