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Optimization of conditions for the in vitro formation of hybrid DNA molecules by DNA ligase

✍ Scribed by Hermann Graf


Book ID
115747293
Publisher
Elsevier Science
Year
1979
Weight
744 KB
Volume
564
Category
Article
ISSN
0005-2787

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πŸ“œ SIMILAR VOLUMES


In vitro selection of optimal DNA substr
✍ Kazuo Harada; Leslie E. Orgel πŸ“‚ Article πŸ“… 1994 πŸ› Springer 🌐 English βš– 279 KB

We have used in vitro selection to investigate the sequence requirements for efficient templatedirected ligation of oligonucleotides at 0Β°C using a water-soluble carbodiimide as condensing agent. We find that only 2 bp at each side of the ligation junction are needed. We also studied chemical ligati