In vitro selection of optimal DNA substrates for ligation by a water-soluble carbodiimide

✍ Scribed by Kazuo Harada; Leslie E. Orgel

• Book ID: 104652414
• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 279 KB
• Volume: 38
• Category: Article
• ISSN: 0022-2844
• DOI: 10.1007/bf00175874

✦ Synopsis

We have used in vitro selection to investigate the sequence requirements for efficient templatedirected ligation of oligonucleotides at 0°C using a water-soluble carbodiimide as condensing agent. We find that only 2 bp at each side of the ligation junction are needed. We also studied chemical ligation of substrate ensembles that we have previously selected as optimal for ligation by RNA ligase or by DNA ligase. As anticipated, we find that substrates selected with DNA ligase ligate efficiently with a chemical ligating agent, and vice versa. Substrates selected using RNA ligase are not ligated by the chemical condensing agent and vice versa. The implications of these results for prebiotic chemistry are discussed.