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Optimization of conditions for electroporation and transient expression of foreign genes in sugarcane protoplasts

โœ Scribed by Carl Rathus; Robert G. Birch


Book ID
119136855
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
749 KB
Volume
81
Category
Article
ISSN
0168-9452

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We have investigated factors influencing polyethylene glycol mediated DNA uptake and B-glucuronidase expression in pea (Pisum sativum L.) protoplasts. It was found that for optimal 13-glucuronidase expression the molecular weight and concentration of polyethylene glycol should be 4000 and 20%, respe