Optimization of polyethylene glycol medi
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Mogens Nicolaisen; Gert B. Poulsen
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Article
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1993
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Springer
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English
โ 357 KB
We have investigated factors influencing polyethylene glycol mediated DNA uptake and B-glucuronidase expression in pea (Pisum sativum L.) protoplasts. It was found that for optimal 13-glucuronidase expression the molecular weight and concentration of polyethylene glycol should be 4000 and 20%, respe