Optimization of Conditions for Collagen Extraction from the Swim Bladders of Grass Carp (Ctenopharyngodon idella) by Response Surface Methodology

✍ Scribed by Zhang, Bao; Chen, Yunzhong; Wei, Xuefei; Li, Mingqi; Wang, Mengjin

• Book ID: 125805114
• Publisher: Walter de Gruyter GmbH & Co. KG
• Year: 2010
• Tongue: English
• Weight: 523 KB
• Volume: 6
• Category: Article
• ISSN: 2194-5764
• DOI: 10.2202/1556-3758.1772

✦ Synopsis

The effects of liquid-solid ratio, acetic acid concentration and extraction time on the yield of acid-soluble collagen(ASC) from the swim bladders of grass carp were optimized by statistical analysis using response surface methodology. The response surface methodology (RSM) was used to optimize the yield of ASC by implementing the Box-Wilson design. Statistical analysis of the results showed that the linear and quadric terms of these three variables had significant effects. However, no interactions between the three variables were found to contribute to the response at a significant level. The optimal conditions for higher yield of ASC were a liquid-solid ratio of 17.85, an acetic-acid concentration of 0.54 M and a time of 34 h. Under these conditions, the model predicted an ASC yield of 8.39%. Verification of the optimization showed that an ASC yield of 8.21±0.15% was observed under the optimal conditions. The experimental values agreed with the predicted values, using analysis of variance, indicating an excellent fit of the model used and the success of response surface methodology for modeling extraction of ASC from the swim bladders of grass carp.