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Optimization of CHO cell culture medium for production of recombinant human erythropoietin and interferon beta-1a

✍ Scribed by Lobanova, Natalya; Savinova, Irina; Trusova, Irina; Kopylova, Olga; Sautkina, Elena; Seregin, Yury


Book ID
122788861
Publisher
Elsevier
Year
2012
Tongue
English
Weight
71 KB
Volume
29
Category
Article
ISSN
1871-6784

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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37Β°C until viable cell concentration reached 1 × 10^7^ c