Optimization of calyculin A-induced premature chromosome condensation assay for chromosome aberration studies
โ Scribed by Tomisato Miura; William F. Blakely
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 331 KB
- Volume
- 79A
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract The phenomenon of premature chromosome condensation (PCC) is induced in unstimulated horse lymphocytes, bovine spermatozoa, Chinese hamster ovary cells, embryonic chick fibroblasts and erythrocytes, __Xenopus__ kidney and mosquito cells by fusing each of these cell types with HeLa cells
It is well established that specific cancers and immortalized cells have nonrandom chromosome aberrations. However, little is understood about the underlying mechanism that initiates these aberrations in human cells. To examine whether human chromosomes with the shortest telomeres initiate the prefe
## Abstract International guidelines for cytotoxicity limits for the in vitro chromosomal aberration assay require reductions in cell growth of greater than 50%. This sets no upper limit on toxicity and there is concern about the number of false or irrelevant results obtained in the aberration assa