A continuous culture technique was used to optimize the medium composition and growth conditions of a mixed bacterial culture utilizing methanol. The improved medium resuited in satisfactory growth, high-yield coefficients and gave a product containing reduced polysaccharide concentrations. Optimal
Optimization of biomass productivity and substrate utility of a hydrogen bacterium, Alcaligenes hydrogenophilus
โ Scribed by Yoshiharu Miura; Mitsuo Okazaki; Kiyomoto Ohi; Takashi Nishimura; Sadao Komemushi
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 355 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0006-3592
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โฆ Synopsis
Optimizations of cell productivity and utility of gaseous substrates were investigated for Alculigenes hydrogenophifus. Theoretical analyses for the cell productivity and substrate utility were discussed in the light of experimental data. It was proved from the theoretical analyses that the maximum cell productivity was obtained at the following conditions: D = 0.21 h-I. R , ( = H2 partial pressure/02 partial pressure) = 2.25 and R 2 ( = C 0 2 partial pressure/02 partial pressure) = 0.09, i.e.. a molal ratio. H2 : 02 : C 0 2 = 67.4: 29.9: 2.7. The condition which gave the maximum cell productivity also gave high utilities of gaseous substrates. The growth yield for oxygen increased with increasing R , in chemostat culture. and the growth yield for hydrogen slightly increased with increasing R,. Theoretical cell productivity and substrate utiliry were in good agreement with experimental values. taking into account the change of growth yield for oxygen.
๐ SIMILAR VOLUMES
The effects of rheological change by addition of sodium carboxymethylcellulose (CMC) to culture medium in an air-lift-type fermentor on autotrophic production of poly-(D-3-hydroxybutyric acid) [P(3HB)] by twostage culture of Alcaligenes eutrophus is investigated. Addition of 0.05% CMC increased P(3H