Chemostat optimization of biomass production of a mixed bacterial culture utilizing methanol

✍ Scribed by A. S. Abu-Ruwaida; I. M. Banat; I. Y. Hamdan

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 636 KB
• Volume: 32
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00173726

✦ Synopsis

A continuous culture technique was used to optimize the medium composition and growth conditions of a mixed bacterial culture utilizing methanol. The improved medium resuited in satisfactory growth, high-yield coefficients and gave a product containing reduced polysaccharide concentrations. Optimal growth and biomass yields occurred at pH 6.8 a temperature of 37°C and dissolved oxygen at >20% saturation. The maximum growth rate was 0.58 h -1 and maximum biomass yield 0.48 g g-1. The protein content of the product ranged between 81%-83%, and nucleic acid content between 10%-12%, increasing with growth rate. The amino acid profile of the mixed culture product m e t and, in some cases, exceeded the UN Food and Agricultural Organization standard, indicating a good source of feed protein.