𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Optimization of Biocatalyst Specific Activity for Glycolic Acid Production

✍ Scribed by Arie Ben-Bassat; Alison M. Walls; Matthew A. Plummer; Amy E. Sigmund; William L. Spillan; Robert DiCosimo

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
280 KB
Volume
350
Category
Article
ISSN
1615-4150

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A chemoenzymatic process has been developed that employs an immobilized microbial nitrilase biocatalyst for the conversion of glycolonitrile to high‐purity glycolic acid. The specific activity of this immobilized cell biocatalyst decreased significantly during initial use in either consecutive batch reactions with catalyst recycle, or in a continuous stirred‐tank reactor, but the nitrilase activity remaining after this initial decrease was stable under the reactions conditions. The initial stability of this immobilized cell nitrilase catalyst has been improved by treatment of the microbial cells with glutaraldehyde prior to immobilization. Conditions for glutaraldehyde treatment were defined that completely inactivated the culture without significantly affecting nitrilase activity. A method for dehydration, storage and rehydration of the carrageenan‐immobilized cells has also been demonstrated that further improves the specific activity of this biocatalyst.

📜 SIMILAR VOLUMES

Protein engineering of nitrilase for che
Protein engineering of nitrilase for chemoenzymatic production of glycolic acid
✍ Shijun Wu; Arthur J. Fogiel; Kelly L. Petrillo; Raymond E. Jackson; Kimberley N. 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 127 KB 👁 1 views

## Abstract A key step in a chemoenzymatic process for the production of high‐purity glycolic acid (GLA) is the enzymatic conversion of glycolonitrile (GLN) to ammonium glycolate using a nitrilase derived from __Acidovorax facilis__ 72W. Protein engineering and over‐expression of this nitrilase, co

Experimental optimization of fed-batch c
Experimental optimization of fed-batch culture for poly-β-hydroxybutyric acid production
✍ Jung Heon Lee; Juan Hong; Henry C. Lim 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 143 KB 👁 1 views

The optimal feed rate profiles of glucose and ammonium hydroxide were calculated using a proposed model, and implemented for the production of poly-␤hydroxybutyric acid (PHB) by Alcaligenes eutrophus. By implementing these optimal feed rates with a high glucose feed concentration of 700 g/L and an a

Optimal design of an integrated fermenta
Optimal design of an integrated fermentation process for lactic acid production
✍ Hsun-Tung Lin; Feng-Sheng Wang 📂 Article 📅 2007 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 176 KB 👁 2 views

## Abstract In this work, we considered a multistage integrated continuous fermentation process for producing lactic acid. Each stage consists of a mixing tank, a bioreactor, a cell‐recycle unit, and an extractor. A generalized mathematical model was formulated to express the integrated process. We