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Experimental optimization of fed-batch culture for poly-β-hydroxybutyric acid production

✍ Scribed by Jung Heon Lee; Juan Hong; Henry C. Lim

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 143 KB
Volume: 56
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19971220)56:6<697::aid-bit13>3.0.co;2-5

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✦ Synopsis

The optimal feed rate profiles of glucose and ammonium hydroxide were calculated using a proposed model, and implemented for the production of poly-␤hydroxybutyric acid (PHB) by Alcaligenes eutrophus. By implementing these optimal feed rates with a high glucose feed concentration of 700 g/L and an ammonium hydroxide concentration of 7%(w/w), it was possible to achieve a high final cell concentration of 141 g/L and a high PHB concentration of 105 g/L in 40 h of fed-batch operation. The PHB productivity was as high as 2.63 g/(L hr).

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