## Abstract The study of large groups of individuals (from reactive arthritis epidemics, for example) for epidemiologic and genetic data has been hampered by the techniques by which HLA typing is performed. Microlympho‐cytotoxicity is costly not only in materials but also in the time required to pe
Optimisation of HLA-B27 Testing by Association of Flow Cytometry and DNA Typing
✍ Scribed by G. Bonnaud; C. Aupetit; P. M. Preux; M. Cogné; M. Drouet
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 48 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0770-3198
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A biannual external quality assurance (EQA) scheme for flow cytometric typing of the HLA-B27 antigen is operational in The Netherlands and Belgium since 1995. We report here on the results of the first seven send-outs to which 36 to 47 laboratories participated. With the send-out, four specimens fro
Typing for HLA-B27 by serological methods is routinely performed using the microlymphocytotoxic test (MLCT). Since monoclonal antibodies (MAB) against HLA-B27 are available, flow cytometry (FC), which requires less time than the MLCT has been developed as an alternative technique. The aim of the pre
The determination of HLA-B27 (827) status is important in the diagnosis of ankylosing spondylitis, Reiter's disease, and other arthropathies. Flow cytometric (FCM) typing of 827 is a relatively new method which allows for rapid turnaround time and low cost. However, different leukocyte populations m
## Abstract ## Background Human leukocyte antigen B27 (HLA‐B27) is a major histocompatibility complex class 1 molecule that is strongly associated with the disease ankylosing spondylitis. Testing for HLA‐B27 is of diagnostic value because 90% of patients with ankylosing spondylitis have the B27 an