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Optimisation of aqueous two-phase extraction of human antibodies

✍ Scribed by Ana M. Azevedo; Paula A.J. Rosa; I. Filipa Ferreira; M. Raquel Aires-Barros


Book ID
118471942
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
603 KB
Volume
132
Category
Article
ISSN
0168-1656

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Amino acids, including lysine, glutamic acid, and phenylalanine, in pure solution or in fermentation broth, were extracted with the aqueous two-phase system consisting of polyethylene glycol and salts, giving a very sharp separation. The partition is influenced by the type and the amount of salts us