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Optimal production of recombinant protein by the baculovirus-insect cell system in shake-flask culture with medium replacement

✍ Scribed by Hideki Yamaji; Shin-Ichi Tagai; Hideki Fukuda


Book ID
114399861
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
698 KB
Volume
87
Category
Article
ISSN
1389-1723

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Shake flasks were successfully employed for the cultivation of Spodoptera frugiperda (Sf-9) insect cells and for the production of beta-galactosidase, a recombinant model protein, utilizing the baculovirus expression vector system. The culture doubling time and maximal cell density were 20 h and 5 x