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Optimal Parameters for the Polybrene-Induced DNA Transfection of Diploid Human Fibroblasts

โœ Scribed by Thomas L. Morgan, Veronica M. Maher and J. Justin McCormick

Book ID
125620476
Publisher
Society for In Vitro Biology
Year
1986
Tongue
English
Weight
163 KB
Volume
22
Category
Article
ISSN
1475-2689

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The cytotoxic effect of a direct perturbation of DNA during various portions of the DNA synthetic period (S phase) of cultured human diploid fibroblasts was examined. The cells were synchronized by a period of growth in low serum with a subsequent blockage of the cells at the GI/S boundary by hydrox