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Optimal parameters for the polybrene-induced DNA transfection of diploid human fibroblasts

โœ Scribed by Thomas L. Morgan; Veronica M. Maher; J. Justin McCormick

Book ID
112799712
Publisher
Society for In Vitro Biology
Year
1986
Tongue
English
Weight
304 KB
Volume
22
Category
Article
ISSN
1475-2689

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The cytotoxic effect of a direct perturbation of DNA during various portions of the DNA synthetic period (S phase) of cultured human diploid fibroblasts was examined. The cells were synchronized by a period of growth in low serum with a subsequent blockage of the cells at the GI/S boundary by hydrox