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Optimal conditions for the toxoiding of pertussis toxin with 1-ethyl-3(3-dimethylaminopropyl) carbodiimide · HCl

✍ Scribed by M. Christodoulides; R. Parton; D.E.S. Stewart-Tull


Book ID
109314636
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
718 KB
Volume
47
Category
Article
ISSN
0378-1097

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Use of water-soluble 1-ethyl-3(3-dimethy
✍ Mikihiko Kobayashi; Eiji Ichishima 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 395 KB

Reaction between glucuronic acid and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was monitored by the o-phthalaldehyde (OPA) method, which was developed for the fluorescent assay of compounds containing an amino group. About 1 nmol of glucuronic acid was detected by this method. This EDC-OPA