✦ LIBER ✦

Use of water-soluble 1-ethyl-3(3-dimethylaminopropyl)carbodiimide for the fluorescent determination of uronic acids and carboxylic acids

✍ Scribed by Mikihiko Kobayashi; Eiji Ichishima

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 395 KB
Volume: 189
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(90)90056-f

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✦ Synopsis

Reaction between glucuronic acid and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was monitored by the o-phthalaldehyde (OPA) method, which was developed for the fluorescent assay of compounds containing an amino group. About 1 nmol of glucuronic acid was detected by this method. This EDC-OPA method was effective in detecting not only acidic sugar but also carboxylic acid. Although the sensitivity of the EDC-OPA method was somewhat lower than that of amino acid determination by OPA, a very simple and convenient assay was attained for compounds containing a carboxyl group.

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