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Optical sectioning by two-pinhole confocal fluorescence microscopy

✍ Scribed by M. Martı́nez-Corral; M.T. Caballero; C. Ibáñez-López; V. Sarafis

Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
236 KB
Volume
34
Category
Article
ISSN
0968-4328

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✦ Synopsis

A two-pinhole axially superresolving confocal fluorescence imaging system is presented. Based on the concept of subtractive incoherent imaging, the system described here is equipped with a zero-focus complex-transmittance pupil filter in one of the collector paths. The optical sectioning capacity of the system is 25% superior to that of a free-pupil one-pinhole instrument.

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