Optical property of bovine plasma albumin between 2 and 82 eV

✍ Scribed by T. Inagaki; R. N. Hamm; E. T. Arakawa; R. D. Birkhoff

• Publisher: Wiley (John Wiley & Sons)
• Year: 1975
• Tongue: English
• Weight: 474 KB
• Volume: 14
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1975.360140412

✦ Synopsis

Abstract

The optical constants of bovine plasma albumin in the form of a dry solid film have been determined in the 2–82 eV region of photon energy. The extinction coefficient k was obtained by transmission measurements on thin films on a calcium fluoride substrate and on ultrathin films of polystyrene supported by mesh screen. The refractive index n was determined by a Kramers–Kronig analysis using the measured values of k. The complex dielectric function ε and the energy‐loss function −Im(1/ε) for charged particles were derived from the data of n and k. The result for k exhibit, in addition to the well‐known absorptions at 4.4, 5.5, and 6.4 eV, a small peak at 7.6 eV, a shoulder around 9.7 eV, and a strong peak at 14.0 eV. A prominent peak at 22.7 eV in the energy‐loss function exhibits a large degree of collective nature.