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Opioid regulation of intracellular free calcium in cultured mouse dorsal root ganglion neurons

✍ Scribed by T. Tang; B.A. Stevens; B.M. Cox

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 609 KB
Volume: 44
Category: Article
ISSN: 0360-4012
DOI: 10.1002/(sici)1097-4547(19960515)44:4<338::aid-jnr4>3.0.co;2-d

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✦ Synopsis

Opioid agonists induced an increase in the intracellular free calcium concentration ([Ca2+Ii) or an inhibition of K+ (25 mM)-stimulated increase in [Ca2+Ii in different subsets of mouse dorsal root ganglion (DRG) neurons. The total neuronal population was grouped into three classes according to somatic diameter and defined as small (<16 pm), intermediate (16-25 pm), or large (>25 pm) neurons. Substance P-like immunoreactivity was detected mainly in the small and intermediate neurons. The 6, K, and p opioid receptor agonists [D-Ser2,Leu5]enkephalin-Thr (DSLET), U69593, and [D-Ala2, MePhe4, Gly-o15]enkephalin (DAMGO) each induced a transient increase in [Ca2+Ii in a small fraction (<30%) of neurons. The increases in [CaZ+li were blocked by the opioid antagonist naloxone. The dihydropyridinesensitive calcium channel blocker nifedipine also blocked the increase in [Ca2+Ii induced by 1 pM DSLET. The rank order of potency (percentage of cells responding to each opioid agonist) was DSLET > U69593 > DAMGO. The opioid-induced increase in [Ca2+Ii was observed mainly in large neurons, with a low incidence in small and intermediate neurons. Opioid agonists also caused inhibition of K+stimulated increases in [Ca2+Ii, which were blocked by naloxone (1 pM). Inhibition of the K+-stimulated increase by 1 pM DSLET or U69593 was greater in small and intermediate neurons than in large neurons. 0

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